Isolation of fluorescein diacetate stained hyphae from soil by micromanipulation

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Abstract

Stained fluorescent hyphae were picked out from soil using a Skerman micromanipulator. About 30% of the plated out hyphae produced visible colonies, at least half of which could be considered to be derived from hyphae, The dominating genera were Penicillium, Oidiodendron and Mortierella. Non-sporulating fungi were rather few (4'5%). Results were
compared with those obtained using the dilution plate and soil washing techniques.

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Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)465-468
JournalTransactions of the British Mycological Society
Volume86
Issue number3
Publication statusPublished - 1986
Publication categoryResearch
Peer-reviewedYes