Ku protein in human T and B lymphocytes: full length functional form and signs of degradation

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Abstract

DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa.

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Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Ku protein, DNA-dependent protein kinase, B lymphocyte, T lymphocyte
Original languageEnglish
Pages (from-to)305-312
JournalBiochimica et biophysica acta
Volume1538
Issue number2-3
Publication statusPublished - 2001
Publication categoryResearch
Peer-reviewedYes