Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

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Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow. / Berclaz, Corinne; Szlag, Daniel; Nguyen, David; Extermann, Jérôme; Bouwens, Arno; Marchand, Paul J.; Nilsson, Julia; Schmidt-Christensen, Anja; Holmberg, Dan; Grapin-Botton, Anne; Lasser, Theo.

In: Biomedical Optics Express, Vol. 7, No. 11, #273588, 01.11.2016, p. 4569-4580.

Research output: Contribution to journalArticle

Harvard

Berclaz, C, Szlag, D, Nguyen, D, Extermann, J, Bouwens, A, Marchand, PJ, Nilsson, J, Schmidt-Christensen, A, Holmberg, D, Grapin-Botton, A & Lasser, T 2016, 'Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow', Biomedical Optics Express, vol. 7, no. 11, #273588, pp. 4569-4580. https://doi.org/10.1364/BOE.7.004569

APA

Berclaz, C., Szlag, D., Nguyen, D., Extermann, J., Bouwens, A., Marchand, P. J., Nilsson, J., Schmidt-Christensen, A., Holmberg, D., Grapin-Botton, A., & Lasser, T. (2016). Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow. Biomedical Optics Express, 7(11), 4569-4580. [#273588]. https://doi.org/10.1364/BOE.7.004569

CBE

MLA

Vancouver

Berclaz C, Szlag D, Nguyen D, Extermann J, Bouwens A, Marchand PJ et al. Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow. Biomedical Optics Express. 2016 Nov 1;7(11):4569-4580. #273588. https://doi.org/10.1364/BOE.7.004569

Author

Berclaz, Corinne ; Szlag, Daniel ; Nguyen, David ; Extermann, Jérôme ; Bouwens, Arno ; Marchand, Paul J. ; Nilsson, Julia ; Schmidt-Christensen, Anja ; Holmberg, Dan ; Grapin-Botton, Anne ; Lasser, Theo. / Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 11. pp. 4569-4580.

RIS

TY - JOUR

T1 - Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

AU - Berclaz, Corinne

AU - Szlag, Daniel

AU - Nguyen, David

AU - Extermann, Jérôme

AU - Bouwens, Arno

AU - Marchand, Paul J.

AU - Nilsson, Julia

AU - Schmidt-Christensen, Anja

AU - Holmberg, Dan

AU - Grapin-Botton, Anne

AU - Lasser, Theo

PY - 2016/11/1

Y1 - 2016/11/1

N2 - In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.

AB - In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.

KW - Biology

KW - Flow diagnostics

KW - Optical coherence tomography

KW - Three-dimensional image acquisition

UR - http://www.scopus.com/inward/record.url?scp=84994545324&partnerID=8YFLogxK

U2 - 10.1364/BOE.7.004569

DO - 10.1364/BOE.7.004569

M3 - Article

C2 - 27895996

AN - SCOPUS:84994545324

VL - 7

SP - 4569

EP - 4580

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 11

M1 - #273588

ER -