Large crystal growth for neutron protein crystallography

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains the underlying principles of these protein crystallization methods and provides practical examples that may help others to successfully prepare large crystals for NPX.

Details

Authors
Organisations
External organisations
  • University Grenoble Alpes
  • European Spallation Source ESS AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Structural Biology

Keywords

  • Dialysis, Microseeding, Phase diagram, Vapor diffusion
Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press
Publication statusE-pub ahead of print - 2020 Jan 21
Publication categoryResearch
Peer-reviewedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988