Lattice model calculations of interactions between proteins and surface grafted polymers with tethered affinity ligands

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Lattice model calculations of interactions between proteins and surface grafted polymers with tethered affinity ligands. / Karlström, Gunnar; Johansson, Hans-Olof.

In: Colloids and Surfaces B: Biointerfaces, Vol. 20, No. 3, 2001, p. 245-256.

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TY - JOUR

T1 - Lattice model calculations of interactions between proteins and surface grafted polymers with tethered affinity ligands

AU - Karlström, Gunnar

AU - Johansson, Hans-Olof

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039), Biochemistry and Structural Biology (S) (000006142)

PY - 2001

Y1 - 2001

N2 - Monte Carlo calculations of protein binding to affinity ligands tethered to a surface by polymers have been done and analyzed with statistical mechanical perturbation theory. The interaction of the polymers with the surface, the solvent and the protein has been varied. Different solution conditions of the polymers have been investigated, varying from collapsed polymer structures on a surface to structures extending out in the solution (athermic condition) or to mushroom like structures (hydrophobic polymers grafted on hydrophilic surface). The variation in binding of model proteins of different sizes and interactions with polymers has been studied. In general, smaller proteins bind better than larger proteins. Two types of polymer collapses have been studied. One type is due to increased polymer-surface attraction. The second type is due to increased polymer-self attraction. In the former case the binding, as a function of degree of collapse, decreases monotonically except for small proteins with attraction to the polymer. For collapses of the second type the loss of binding goes through a maximum except for large proteins.

AB - Monte Carlo calculations of protein binding to affinity ligands tethered to a surface by polymers have been done and analyzed with statistical mechanical perturbation theory. The interaction of the polymers with the surface, the solvent and the protein has been varied. Different solution conditions of the polymers have been investigated, varying from collapsed polymer structures on a surface to structures extending out in the solution (athermic condition) or to mushroom like structures (hydrophobic polymers grafted on hydrophilic surface). The variation in binding of model proteins of different sizes and interactions with polymers has been studied. In general, smaller proteins bind better than larger proteins. Two types of polymer collapses have been studied. One type is due to increased polymer-surface attraction. The second type is due to increased polymer-self attraction. In the former case the binding, as a function of degree of collapse, decreases monotonically except for small proteins with attraction to the polymer. For collapses of the second type the loss of binding goes through a maximum except for large proteins.

KW - Surface grafted polymer

KW - Affinity ligand

KW - Lattice model

KW - Protein

U2 - 10.1016/S0927-7765(00)00199-5

DO - 10.1016/S0927-7765(00)00199-5

M3 - Article

VL - 20

SP - 245

EP - 256

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 1873-4367

IS - 3

ER -