LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects

Research output: Contribution to journalArticle

Standard

LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects. / Zhang, Botao; Carrie, Chris; Ivanova, Aneta; Narsai, Reena; Murcha, Monika W; Duncan, Owen; Wang, Yan; Law, Simon R; Albrecht, Verónica; Pogson, Barry James; Giraud, Estelle; Van Aken, Olivier; Whelan, James.

In: Journal of Biological Chemistry, Vol. 287, No. 50, 07.12.2012, p. 41757-73.

Research output: Contribution to journalArticle

Harvard

Zhang, B, Carrie, C, Ivanova, A, Narsai, R, Murcha, MW, Duncan, O, Wang, Y, Law, SR, Albrecht, V, Pogson, BJ, Giraud, E, Van Aken, O & Whelan, J 2012, 'LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects', Journal of Biological Chemistry, vol. 287, no. 50, pp. 41757-73. https://doi.org/10.1074/jbc.M112.383836

APA

Zhang, B., Carrie, C., Ivanova, A., Narsai, R., Murcha, M. W., Duncan, O., Wang, Y., Law, S. R., Albrecht, V., Pogson, B. J., Giraud, E., Van Aken, O., & Whelan, J. (2012). LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects. Journal of Biological Chemistry, 287(50), 41757-73. https://doi.org/10.1074/jbc.M112.383836

CBE

MLA

Vancouver

Author

Zhang, Botao ; Carrie, Chris ; Ivanova, Aneta ; Narsai, Reena ; Murcha, Monika W ; Duncan, Owen ; Wang, Yan ; Law, Simon R ; Albrecht, Verónica ; Pogson, Barry James ; Giraud, Estelle ; Van Aken, Olivier ; Whelan, James. / LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 50. pp. 41757-73.

RIS

TY - JOUR

T1 - LETM proteins play a role in the accumulation of mitochondrially encoded proteins in Arabidopsis thaliana and AtLETM2 displays parent of origin effects

AU - Zhang, Botao

AU - Carrie, Chris

AU - Ivanova, Aneta

AU - Narsai, Reena

AU - Murcha, Monika W

AU - Duncan, Owen

AU - Wang, Yan

AU - Law, Simon R

AU - Albrecht, Verónica

AU - Pogson, Barry James

AU - Giraud, Estelle

AU - Van Aken, Olivier

AU - Whelan, James

PY - 2012/12/7

Y1 - 2012/12/7

N2 - The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.

AB - The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). Inactivation of both genes, Atletm1 and Atletm2, together is lethal. Plants that are hemizygous for AtLETM2 and homozygous for Atletm1 (letm1(-/-) LETM2(+/-)) displayed a mild retarded growth phenotype during early seedling growth. It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. Examination of respiratory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that the steady state level of ATP synthase was reduced in abundance, whereas the steady state levels of other respiratory chain proteins remained unchanged. The absence of a functional maternal AtLETM2 allele in an Atletm1 mutant background resulted in early seed abortion. Reciprocal crosses revealed that maternally, but not paternally, derived AtLETM2 was absolutely required for seed development. This requirement for a functional maternal allele of AtLETM2 was confirmed using direct sequencing of reciprocal crosses of Col-0 and Ler accessions. Furthermore, AtLETM2 promoter β-glucuronidase constructs displayed exclusive maternal expression patterns.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Electron Transport

KW - Gene Expression Regulation, Plant

KW - Mitochondria

KW - Mitochondrial Proteins

U2 - 10.1074/jbc.M112.383836

DO - 10.1074/jbc.M112.383836

M3 - Article

C2 - 23043101

VL - 287

SP - 41757

EP - 41773

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 1083-351X

IS - 50

ER -