Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals

Research output: Contribution to journalArticle

Abstract

Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids. Here we identify the linoleic acid ester of 13-hydroxy linoleic acid (13-LAHLA) as an anti-inflammatory lipid. An oat oil fraction and FAHFA-enriched extract from this fraction showed anti-inflammatory activity in a lipopolysaccharide-induced cytokine secretion assay. Structural studies identified three LAHLA isomers (15-, 13-, and 9-LAHLA) as being the most abundant FAHFAs in the oat oil fraction. Of these LAHLAs, 13-LAHLA is the most abundant LAHLA isomer in human serum after ingestion of liposomes made of fractionated oat oil, and it is also the most abundant endogenous LAHLA in mouse and human adipose tissue. As a result, we chemically synthesized 13-LAHLA for biological assays. 13-LAHLA suppresses lipopolysaccharide-stimulated secretion of cytokines and expression of pro-inflammatory genes. These studies identify LAHLAs as an evolutionarily conserved lipid with anti-inflammatory activity in mammalian cells.

Details

Authors
  • Matthew J. Kolar
  • Srihari Konduri
  • Tina Chang
  • Huijing Wang
  • Clare McNerlin
  • Lena Ohlsson
  • Magnus Härröd
  • Dionicio Siegel
  • Alan Saghatelian
Organisations
External organisations
  • University of California, San Diego
  • Salk Institute for Biological Studies
  • Härröd Research AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
Original languageEnglish
Pages (from-to)10698-10707
Number of pages10
JournalJournal of Biological Chemistry
Volume294
Issue number27
Publication statusPublished - 2019 Jul 5
Publication categoryResearch
Peer-reviewedYes