Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain

Research output: Contribution to journalArticle

Abstract

Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide.

Details

Authors
External organisations
  • Hagedorn Research Institute
Research areas and keywords

Keywords

  • ELISA, Flow cytofluorometry, HLA class II antigen, Immunoblotting bl]
Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalFEBS Letters
Volume189
Issue number2
Publication statusPublished - 1985 Sep 23
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes