Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain

Research output: Contribution to journalArticle

Bibtex

@article{613a4ea736ea4a06b674f30eb6be5577,
title = "Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain",
abstract = "Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide.",
keywords = "ELISA, Flow cytofluorometry, HLA class II antigen, Immunoblotting bl]",
author = "Thomas Deufel and Anne Grove and Hans Kofod and {\AA}ke Lernmark",
year = "1985",
month = sep,
day = "23",
doi = "10.1016/0014-5793(85)81050-4",
language = "English",
volume = "189",
pages = "329--337",
journal = "FEBS Letters",
issn = "1873-3468",
publisher = "Wiley-Blackwell",
number = "2",

}