Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects

Research output: Contribution to journalArticle


Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH2 and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology
Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBiotechnology Letters
Issue number1
Publication statusPublished - 1997 Mar 8
Publication categoryResearch