Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects

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Abstract

Immobilized α-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH2 and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.

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Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology
Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBiotechnology Letters
Volume19
Issue number1
Publication statusPublished - 1997 Mar 8
Publication categoryResearch
Peer-reviewedYes