Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow.

Research output: Contribution to journalArticle

Abstract

Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)965-974
JournalStem Cell Reports
Volume3
Issue number6
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available

Related research output

Dimitra Zacharaki, 2018, Lund: Lund University: Faculty of Medicine. 91 p.

Research output: ThesisDoctoral Thesis (compilation)

Roshanak Ghazanfari, 2017, Lund: Lund University: Faculty of Medicine. 78 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (2)