Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior

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Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior. / Abreu, Soraia Carvalho; Enes, Sara Rolandsson; Dearborn, Jacob; Goodwin, Meagan; Coffey, Amy; Borg, Zachary D; Dos Santos, Claudia C; Wargo, Matthew J; Cruz, Fernanda Ferreira; Loi, Roberto; DeSarno, Michael; Ashikaga, Takamaru; Antunes, Mariana Alves; Rocco, Patricia R M; Liu, Kathleen D; Lee, Jae-Woo; Matthay, Michael A; McKenna, D H; Weiss, Daniel J.

In: American Journal of Physiology: Lung Cellular and Molecular Physiology, Vol. 317, No. 6, 01.12.2019, p. L823-L831.

Research output: Contribution to journalArticle

Harvard

Abreu, SC, Enes, SR, Dearborn, J, Goodwin, M, Coffey, A, Borg, ZD, Dos Santos, CC, Wargo, MJ, Cruz, FF, Loi, R, DeSarno, M, Ashikaga, T, Antunes, MA, Rocco, PRM, Liu, KD, Lee, J-W, Matthay, MA, McKenna, DH & Weiss, DJ 2019, 'Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior', American Journal of Physiology: Lung Cellular and Molecular Physiology, vol. 317, no. 6, pp. L823-L831. https://doi.org/10.1152/ajplung.00263.2019

APA

CBE

Abreu SC, Enes SR, Dearborn J, Goodwin M, Coffey A, Borg ZD, Dos Santos CC, Wargo MJ, Cruz FF, Loi R, DeSarno M, Ashikaga T, Antunes MA, Rocco PRM, Liu KD, Lee J-W, Matthay MA, McKenna DH, Weiss DJ. 2019. Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior. American Journal of Physiology: Lung Cellular and Molecular Physiology. 317(6):L823-L831. https://doi.org/10.1152/ajplung.00263.2019

MLA

Vancouver

Author

Abreu, Soraia Carvalho ; Enes, Sara Rolandsson ; Dearborn, Jacob ; Goodwin, Meagan ; Coffey, Amy ; Borg, Zachary D ; Dos Santos, Claudia C ; Wargo, Matthew J ; Cruz, Fernanda Ferreira ; Loi, Roberto ; DeSarno, Michael ; Ashikaga, Takamaru ; Antunes, Mariana Alves ; Rocco, Patricia R M ; Liu, Kathleen D ; Lee, Jae-Woo ; Matthay, Michael A ; McKenna, D H ; Weiss, Daniel J. / Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior. In: American Journal of Physiology: Lung Cellular and Molecular Physiology. 2019 ; Vol. 317, No. 6. pp. L823-L831.

RIS

TY - JOUR

T1 - Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior

AU - Abreu, Soraia Carvalho

AU - Enes, Sara Rolandsson

AU - Dearborn, Jacob

AU - Goodwin, Meagan

AU - Coffey, Amy

AU - Borg, Zachary D

AU - Dos Santos, Claudia C

AU - Wargo, Matthew J

AU - Cruz, Fernanda Ferreira

AU - Loi, Roberto

AU - DeSarno, Michael

AU - Ashikaga, Takamaru

AU - Antunes, Mariana Alves

AU - Rocco, Patricia R M

AU - Liu, Kathleen D

AU - Lee, Jae-Woo

AU - Matthay, Michael A

AU - McKenna, D H

AU - Weiss, Daniel J

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent effects on mRNA expression of selected pro- and anti-inflammatory mediators, and for overall patterns of gene and mRNA expression. Both common and disease specific-patterns were observed in gene expression of different hMSC mediators, notably interleukin (IL)-6. Conditioned media obtained from non-ARDS BALF-exposed hMSCs was more effective in promoting an anti-inflammatory phenotype in monocytes than was conditioned media from ARDS BALF-exposed hMSCs. Neutralizing IL-6 in the conditioned media promoted generation of anti-inflammatory monocyte phenotype. These results demonstrated that different lung inflammatory environments differentially alter hMSC behavior. Further identification of these interactions and the driving mechanisms may influence clinical use of MSCs for treating lung diseases.

AB - Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent effects on mRNA expression of selected pro- and anti-inflammatory mediators, and for overall patterns of gene and mRNA expression. Both common and disease specific-patterns were observed in gene expression of different hMSC mediators, notably interleukin (IL)-6. Conditioned media obtained from non-ARDS BALF-exposed hMSCs was more effective in promoting an anti-inflammatory phenotype in monocytes than was conditioned media from ARDS BALF-exposed hMSCs. Neutralizing IL-6 in the conditioned media promoted generation of anti-inflammatory monocyte phenotype. These results demonstrated that different lung inflammatory environments differentially alter hMSC behavior. Further identification of these interactions and the driving mechanisms may influence clinical use of MSCs for treating lung diseases.

UR - https://scopus.com/record/display.uri?eid=2-s2.0-85076125829&origin=inward&txGid

U2 - 10.1152/ajplung.00263.2019

DO - 10.1152/ajplung.00263.2019

M3 - Article

VL - 317

SP - L823-L831

JO - American Journal of Physiology: Lung Cellular and Molecular Physiology

JF - American Journal of Physiology: Lung Cellular and Molecular Physiology

SN - 1522-1504

IS - 6

ER -