Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

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@article{2f33c9a6540f4ebaa9c3139a17b4de63,
title = "Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism",
abstract = "Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.",
keywords = "Animals, Diet, Forkhead Transcription Factors/immunology, Homeostasis/immunology, Interleukin-17/immunology, Intestine, Large/immunology, Intestine, Small/immunology, Male, Methylococcus capsulatus/chemistry, Mice, Inbred C57BL, Microbiota/immunology, Nuclear Receptor Subfamily 1, Group F, Member 3/immunology, Obesity/immunology, Proteins/immunology, T-Lymphocytes, Regulatory/immunology",
author = "Jensen, {Benjamin A H} and Holm, {Jacob B} and Larsen, {Ida S} and {von Burg}, Nicole and Stefanie Derer and Sonne, {Si B} and P{\ae}rregaard, {Simone I} and Damgaard, {Mads V} and Indrelid, {Stine A} and Aymeric Rivollier and Anne-Laure Agrinier and Karolina Sulek and Arnoldussen, {Yke J} and Even Fj{\ae}re and Andr{\'e} Marette and Angell, {Inga L} and Knut Rudi and Treebak, {Jonas T} and Lise Madsen and {\AA}kesson, {Caroline Piercey} and William Agace and Christian Sina and Kleiveland, {Charlotte R} and Karsten Kristiansen and Lea, {Tor E}",
note = "These authors jointly supervised this work: Benjamin A. H. Jensen, Karsten Kristiansen, Tor E. Lea.",
year = "2021",
month = feb,
day = "17",
doi = "10.1038/s41467-021-21408-9",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}