Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

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Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism. / Jensen, Benjamin A H; Holm, Jacob B; Larsen, Ida S; von Burg, Nicole; Derer, Stefanie; Sonne, Si B; Pærregaard, Simone I; Damgaard, Mads V; Indrelid, Stine A; Rivollier, Aymeric; Agrinier, Anne-Laure; Sulek, Karolina; Arnoldussen, Yke J; Fjære, Even; Marette, André; Angell, Inga L; Rudi, Knut; Treebak, Jonas T; Madsen, Lise; Åkesson, Caroline Piercey; Agace, William; Sina, Christian; Kleiveland, Charlotte R; Kristiansen, Karsten; Lea, Tor E.

In: Nature Communications, Vol. 12, No. 1, 1093, 17.02.2021.

Research output: Contribution to journalArticle

Harvard

Jensen, BAH, Holm, JB, Larsen, IS, von Burg, N, Derer, S, Sonne, SB, Pærregaard, SI, Damgaard, MV, Indrelid, SA, Rivollier, A, Agrinier, A-L, Sulek, K, Arnoldussen, YJ, Fjære, E, Marette, A, Angell, IL, Rudi, K, Treebak, JT, Madsen, L, Åkesson, CP, Agace, W, Sina, C, Kleiveland, CR, Kristiansen, K & Lea, TE 2021, 'Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism', Nature Communications, vol. 12, no. 1, 1093. https://doi.org/10.1038/s41467-021-21408-9

APA

Jensen, B. A. H., Holm, J. B., Larsen, I. S., von Burg, N., Derer, S., Sonne, S. B., Pærregaard, S. I., Damgaard, M. V., Indrelid, S. A., Rivollier, A., Agrinier, A-L., Sulek, K., Arnoldussen, Y. J., Fjære, E., Marette, A., Angell, I. L., Rudi, K., Treebak, J. T., Madsen, L., ... Lea, T. E. (2021). Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism. Nature Communications, 12(1), [1093]. https://doi.org/10.1038/s41467-021-21408-9

CBE

Jensen BAH, Holm JB, Larsen IS, von Burg N, Derer S, Sonne SB, Pærregaard SI, Damgaard MV, Indrelid SA, Rivollier A, Agrinier A-L, Sulek K, Arnoldussen YJ, Fjære E, Marette A, Angell IL, Rudi K, Treebak JT, Madsen L, Åkesson CP, Agace W, Sina C, Kleiveland CR, Kristiansen K, Lea TE. 2021. Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism. Nature Communications. 12(1):Article 1093. https://doi.org/10.1038/s41467-021-21408-9

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Jensen, Benjamin A H ; Holm, Jacob B ; Larsen, Ida S ; von Burg, Nicole ; Derer, Stefanie ; Sonne, Si B ; Pærregaard, Simone I ; Damgaard, Mads V ; Indrelid, Stine A ; Rivollier, Aymeric ; Agrinier, Anne-Laure ; Sulek, Karolina ; Arnoldussen, Yke J ; Fjære, Even ; Marette, André ; Angell, Inga L ; Rudi, Knut ; Treebak, Jonas T ; Madsen, Lise ; Åkesson, Caroline Piercey ; Agace, William ; Sina, Christian ; Kleiveland, Charlotte R ; Kristiansen, Karsten ; Lea, Tor E. / Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism. In: Nature Communications. 2021 ; Vol. 12, No. 1.

RIS

TY - JOUR

T1 - Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

AU - Jensen, Benjamin A H

AU - Holm, Jacob B

AU - Larsen, Ida S

AU - von Burg, Nicole

AU - Derer, Stefanie

AU - Sonne, Si B

AU - Pærregaard, Simone I

AU - Damgaard, Mads V

AU - Indrelid, Stine A

AU - Rivollier, Aymeric

AU - Agrinier, Anne-Laure

AU - Sulek, Karolina

AU - Arnoldussen, Yke J

AU - Fjære, Even

AU - Marette, André

AU - Angell, Inga L

AU - Rudi, Knut

AU - Treebak, Jonas T

AU - Madsen, Lise

AU - Åkesson, Caroline Piercey

AU - Agace, William

AU - Sina, Christian

AU - Kleiveland, Charlotte R

AU - Kristiansen, Karsten

AU - Lea, Tor E

N1 - These authors jointly supervised this work: Benjamin A. H. Jensen, Karsten Kristiansen, Tor E. Lea.

PY - 2021/2/17

Y1 - 2021/2/17

N2 - Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.

AB - Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.

KW - Animals

KW - Diet

KW - Forkhead Transcription Factors/immunology

KW - Homeostasis/immunology

KW - Interleukin-17/immunology

KW - Intestine, Large/immunology

KW - Intestine, Small/immunology

KW - Male

KW - Methylococcus capsulatus/chemistry

KW - Mice, Inbred C57BL

KW - Microbiota/immunology

KW - Nuclear Receptor Subfamily 1, Group F, Member 3/immunology

KW - Obesity/immunology

KW - Proteins/immunology

KW - T-Lymphocytes, Regulatory/immunology

U2 - 10.1038/s41467-021-21408-9

DO - 10.1038/s41467-021-21408-9

M3 - Article

C2 - 33597537

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1093

ER -