Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds

Research output: Contribution to journalArticle

Abstract

Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.

Details

Authors
  • W. Noppe
  • Fatima Plieva
  • K. Vanhoorelbeke
  • H. Deckmyn
  • M. Tuncel
  • A. Tuncel
  • Igor Galaev
  • Bo Mattiasson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • von Willebrand factor, Lactoferrin, Protein purification, Bacteriophages, Cryogel, Macroporous hydrogel
Original languageEnglish
Pages (from-to)293-299
JournalJournal of Biotechnology
Volume131
Issue number3
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes