Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds

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Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds. / Noppe, W.; Plieva, Fatima; Vanhoorelbeke, K.; Deckmyn, H.; Tuncel, M.; Tuncel, A.; Galaev, Igor; Mattiasson, Bo.

In: Journal of Biotechnology, Vol. 131, No. 3, 2007, p. 293-299.

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TY - JOUR

T1 - Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds

AU - Noppe, W.

AU - Plieva, Fatima

AU - Vanhoorelbeke, K.

AU - Deckmyn, H.

AU - Tuncel, M.

AU - Tuncel, A.

AU - Galaev, Igor

AU - Mattiasson, Bo

PY - 2007

Y1 - 2007

N2 - Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.

AB - Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.

KW - von Willebrand factor

KW - Lactoferrin

KW - Protein purification

KW - Bacteriophages

KW - Cryogel

KW - Macroporous hydrogel

U2 - 10.1016/j.jbiotec.2007.06.021

DO - 10.1016/j.jbiotec.2007.06.021

M3 - Article

VL - 131

SP - 293

EP - 299

JO - Journal of Biotechnology

T2 - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 1873-4863

IS - 3

ER -