Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate

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Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate. / Gallagher, Ferdia A; Kettunen, Mikko I. ; Day, Sam E; Hu, De-En; Ardenkjaer-Larsen, Jan-Henrik; Zandt, René in 't; Jensen, Pernille R; Karlsson, Magnus; Golman, Klaes; Lerche, Mathilde H; Brindle, Kevin M.

In: Nature, Vol. 453, No. 7197, 12.06.2008, p. 940-3.

Research output: Contribution to journalArticle

Harvard

Gallagher, FA, Kettunen, MI, Day, SE, Hu, D-E, Ardenkjaer-Larsen, J-H, Zandt, RI, Jensen, PR, Karlsson, M, Golman, K, Lerche, MH & Brindle, KM 2008, 'Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate', Nature, vol. 453, no. 7197, pp. 940-3. https://doi.org/10.1038/nature07017

APA

Gallagher, F. A., Kettunen, M. I., Day, S. E., Hu, D-E., Ardenkjaer-Larsen, J-H., Zandt, R. I. ., ... Brindle, K. M. (2008). Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate. Nature, 453(7197), 940-3. https://doi.org/10.1038/nature07017

CBE

Gallagher FA, Kettunen MI, Day SE, Hu D-E, Ardenkjaer-Larsen J-H, Zandt RI, Jensen PR, Karlsson M, Golman K, Lerche MH, Brindle KM. 2008. Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate. Nature. 453(7197):940-3. https://doi.org/10.1038/nature07017

MLA

Vancouver

Gallagher FA, Kettunen MI, Day SE, Hu D-E, Ardenkjaer-Larsen J-H, Zandt RI et al. Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate. Nature. 2008 Jun 12;453(7197):940-3. https://doi.org/10.1038/nature07017

Author

Gallagher, Ferdia A ; Kettunen, Mikko I. ; Day, Sam E ; Hu, De-En ; Ardenkjaer-Larsen, Jan-Henrik ; Zandt, René in 't ; Jensen, Pernille R ; Karlsson, Magnus ; Golman, Klaes ; Lerche, Mathilde H ; Brindle, Kevin M. / Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate. In: Nature. 2008 ; Vol. 453, No. 7197. pp. 940-3.

RIS

TY - JOUR

T1 - Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate

AU - Gallagher, Ferdia A

AU - Kettunen, Mikko I.

AU - Day, Sam E

AU - Hu, De-En

AU - Ardenkjaer-Larsen, Jan-Henrik

AU - Zandt, René in 't

AU - Jensen, Pernille R

AU - Karlsson, Magnus

AU - Golman, Klaes

AU - Lerche, Mathilde H

AU - Brindle, Kevin M

PY - 2008/6/12

Y1 - 2008/6/12

N2 - As alterations in tissue pH underlie many pathological processes, the capability to image tissue pH in the clinic could offer new ways of detecting disease and response to treatment. Dynamic nuclear polarization is an emerging technique for substantially increasing the sensitivity of magnetic resonance imaging experiments. Here we show that tissue pH can be imaged in vivo from the ratio of the signal intensities of hyperpolarized bicarbonate (H(13)CO(3)(-)) and (13)CO(2) following intravenous injection of hyperpolarized H(13)CO(3)(-). The technique was demonstrated in a mouse tumour model, which showed that the average tumour interstitial pH was significantly lower than the surrounding tissue. Given that bicarbonate is an endogenous molecule that can be infused in relatively high concentrations into patients, we propose that this technique could be used clinically to image pathological processes that are associated with alterations in tissue pH, such as cancer, ischaemia and inflammation.

AB - As alterations in tissue pH underlie many pathological processes, the capability to image tissue pH in the clinic could offer new ways of detecting disease and response to treatment. Dynamic nuclear polarization is an emerging technique for substantially increasing the sensitivity of magnetic resonance imaging experiments. Here we show that tissue pH can be imaged in vivo from the ratio of the signal intensities of hyperpolarized bicarbonate (H(13)CO(3)(-)) and (13)CO(2) following intravenous injection of hyperpolarized H(13)CO(3)(-). The technique was demonstrated in a mouse tumour model, which showed that the average tumour interstitial pH was significantly lower than the surrounding tissue. Given that bicarbonate is an endogenous molecule that can be infused in relatively high concentrations into patients, we propose that this technique could be used clinically to image pathological processes that are associated with alterations in tissue pH, such as cancer, ischaemia and inflammation.

KW - Acid-Base Equilibrium

KW - Animals

KW - Bicarbonates

KW - Carbon Dioxide

KW - Carbon Isotopes

KW - Carbonic Anhydrases

KW - Catalysis

KW - Hydrogen-Ion Concentration

KW - Lymphoma

KW - Magnetic Resonance Imaging

KW - Mice

KW - Neoplasm Transplantation

KW - Phantoms, Imaging

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/nature07017

DO - 10.1038/nature07017

M3 - Article

VL - 453

SP - 940

EP - 943

JO - Nature

T2 - Nature

JF - Nature

SN - 0028-0836

IS - 7197

ER -