Magnetoliposomes as Contrast Agents for Longitudinal in vivo Assessment of Transplanted Pancreatic Islets in a Diabetic Rat Model

Research output: Contribution to journalArticle

Abstract

Magnetoliposomes (MLs) were synthesized and tested for longitudinal monitoring of transplanted pancreatic islets using magnetic resonance imaging (MRI) in rat models. The rat insulinoma cell line INS-1E and isolated pancreatic islets from outbred and inbred rats were used to optimize labeling conditions in vitro. Strong MRI contrast was generated by islets exposed to 50 µg Fe/ml for 24 hours without any increased cell death, loss of function or other signs of toxicity. In vivo experiments showed that pancreatic islets (50-1000 units) labeled with MLs were detectable for up to 6 weeks post-transplantation in the kidney subcapsular space. Islets were also monitored for two weeks following transplantation through the portal vein of the liver. Hereby, islets labeled with MLs and transplanted under the left kidney capsule were able to correct hyperglycemia and had stable MRI signals until nephrectomy. Interestingly, in vivo MRI of streptozotocin induced diabetic rats transplanted with allogeneic islets demonstrated loss of MRI contrast between 7-16 days, indicative of loss of islet structure. MLs used in this study were not only beneficial for monitoring the location of transplanted islets in vivo with high sensitivity but also reported on islet integrity and hereby indirectly on islet function and rejection.

Details

Authors
  • Rita Sofia Garcia Ribeiro
  • Conny Gysemans
  • João Paulo Monteiro Carvalho Mori da Cunha
  • Bella B Manshian
  • Daniel Jirak
  • Jan Kriz
  • Juan Gallo
  • Manuel Bañobre-López
  • Tom Struys
  • Marcel De Cuyper
  • Chantal Mathieu
  • Stefaan J Soenen
  • Willy Gsell
  • Uwe Himmelreich
External organisations
  • Catholic University of Leuven
  • Charles University in Prague
  • Institute for Clinical and Experimental Medicine (IKEM)
  • Hasselt University
  • International Iberian Nanotechnology Laboratory
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Article number11487
Number of pages12
JournalScientific Reports
Volume8
Issue number1
Publication statusPublished - 2018 Jul 31
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes