β-Mannan degradation by gut bacteria - Characterization of β-mannanases from families GH5 and GH26
Research output: Thesis › Doctoral Thesis (compilation)
In Paper I, we have studied the effect of galactomannan on metabolic markers and four bacterial genera in rats fed with guar gum. Guar gum of different viscosities was tested. We found that Bifidobacterium counts were increased when the rats were fed guar gum, regardless of viscosity, while the number of Bacteroides was not different from the control. In Papers II-IV we characterized four enzymes from these genera possibly involved in the degradation of guar gum and other mannans. We characterized BaMan26A, a GH26 β-mannanase from Bifidobacterium adolescentis in Paper II and a GH5 β-mannanase, BlMan5_8, from Bifidobacterium animalis subsp. lactis in Paper IV. In Paper III we characterized two GH26 β-mannanases, BoMan26A and BoMan26B, from Bacteroides ovatus that are encoded by a polysaccharide utilization locus. A crystal structure of BoMan26A displayed the (α/β)8 fold of clan GH-A enzymes and a substrate binding cleft. The four β-mannanases were found to vary in product profile and fine-tuned substrate specificity within the group of β-mannans. While BlMan5_8 produced oligosaccharides of varying length from β-mannan, BoMan26A produced almost exclusively mannobiose from β-mannan. BoMan26B produced mainly mannobiose, while BaMan26A produced mannotriose as a major product. BlMan5_8 is predicted to be secreted, while BoMan26B and BaMan26A appear to be anchored to the cell. BaMan26A is predicted to be located in the periplasmic space.
This work gives further insight in the molecular details of β-mannan catabolism in the gut.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Award date||2015 May 22|
|Publication status||Published - 2015|
Patrick Adlercreutz, Irini Lazou Ahrén, Siv Ahrné, Said Alhamimi, Kristina E Andersson, Kristina E Andersson, Anna Månberger, Ulrika Axling, Ulrika Axling, Björn Bergenståhl, Karin Berger, Inger Björck, Camilla Bränning, Fredrik Bäckhed, Yoghatama Cindya Zanzer, Anders Danielsson, Birgitta Danielsson, Eva Degerman, Petr Dejmek, Estera Dey, Anestis Dougkas, Linda Ekström, Ann-Charlotte Eliasson, Christer Fahlgren, Peter Falck, Peter Falck, Tannaz Ghaffarzadegan, Yvonne Granfeldt, Carl Grey, Ulrika Gunnerud, Åsa Håkansson, Åsa Håkansson, Frida Hållenius, Frida Hållenius, Lina Haskå, Lina Haskå, Emilia Heimann, Per Hellstrand, Lovisa Heyman, Cecilia Holm Wallenberg, Ann-Kristin Holmén-Pålbrink, Olle Holst, Tina Immerstrand, Peter Immerzeel, Greta Jakobsdottir, Bengt Jeppsson, Elin Johansson, Maria Johansson, Maria Johansson, Margareta Johansson, Ulla Johansson, Helena Jones, E N Karlsson, Petia Kovatcheva-Datchary, Evelina Kulcinskaja, Mona Landin-Olsson, Caroline Linninge, Ali Marefati, Nittaya Marungruang, Göran Molin, Anne Nilsson, Einar Nilsson, Ulf Nilsson, Margareta Nyman, Eva Ohlson, Crister Olsson, Rickard Öste, Elin Östman, Lisbeth Persson, Stefan Persson, Merichel Plaza, Olena Prykhodko, Karl Radeborg, Marilyn Rayner, Liza Rosén, Margareta Sandahl, Jonna Sandberg, Malin Sjöö, Kerstin Skog, Peter Spégel, Henrik Stålbrand, Olov Sterner, Julia Svensson, Eden Tareke, Juscelino Tovar, Charlotta Turner, Björn Weström, Jie Xu & Yadong Zhong
2007/07/01 → 2018/01/31
Project: Research › Interdisciplinary research