Mannanase Man26A from Cellulomonas fimi has a mannan-binding module

Research output: Contribution to journalArticle

Abstract

A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The Kd for binding of Man26Abm to locust bean gum (LBG) is ~0.2 μM. Man26A is the first mannanase reported to contain a mannan-binding module.

Details

Authors
  • Dominik Stoll
  • Alisdair Boraston
  • Henrik Stålbrand
  • Bradley W McLean
  • Douglas G Kilburn
  • R Anthony J Warren
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Cellulomonas fimi, Mannanase, Mannan-binding module, Affinity gel electrophoresis
Original languageEnglish
Pages (from-to)265-269
JournalFEMS Microbiology Letters
Volume183
Issue number2
Publication statusPublished - 2000
Publication categoryResearch
Peer-reviewedYes