Mapping the cell binding site on high molecular weight kininogen domain 5

Research output: Contribution to journalArticle

Abstract

Investigations mapped the region(s) on the light chain of high molecular weight kininogen (HK) that participates in cell binding. Sequential and overlapping peptides of domain 5 (D5(H)) were synthesized to determine its cell binding site(s). Three peptides from non-overlapping regions on D5(H) were found to inhibit biotin-HK binding to endothelial cells. Peptides GKE19 and HNL21 weakly inhibited biotin-HK binding with IC50 of 792 and 215 μM, respectively. Peptide HKH20 inhibited biotin-HK binding with an IC50 of 0.2 μM. Two peptides, GGH18 and HVL24, which overlapped HKH20, also inhibited biotin-HK binding to endothelial cells with IC50 values of 108 and 0.8 μM, respectively. Biotinylated HKH20 directly bound to endothelial cells. HK and HKH20 bound at or near the same site on endothelial cells because HK inhibited biotin-HKH20 binding (IC50 = 0.2 μM). A polyclonal anti-HKH20 antibody also blocked biotin-HK binding. Peptides HKH20 and HVL24 and anti- HKH20 antibody also inhibited the procoagulant activity of plasma HK. These data indicated that the cell and artificial surface binding sites on D5(H) overlap. The orientation of HK on endothelial cells may be critical for the assembly and activation of contact system enzymes and the expression of kininogen's anti-thrombin activity.

Details

Authors
  • A. A.K. Hasan
  • D. B. Cines
  • H. Herwald
  • A. H. Schmaier
  • W. Muller-Esterl
External organisations
  • University of Pennsylvania
  • University of Mainz
  • University of Michigan
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages (from-to)19256-19261
JournalJournal of Biological Chemistry
Volume270
Issue number33
Publication statusPublished - 1995 Aug 18
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes