Mass spectrometric determination of selected microbial constituents using fused silica and chiral glass capillary gas chromatography

Research output: Contribution to journalArticle

Abstract

Applications of quantitative gas chromatography/mass spectrometry (selected ion monitoring) to clinical and ecological microbiology are described. The technique permits determination of selected microbial metabolites and can be used for the rapid diagnosis of, for example, tuberculosis by ion-focusing of specific branch-chain fatty acids. The technique is also used to determine diaminopimelic and muramic acids, and D- and L-amino acids separated by chiral gas chromatography, in the low picogram range. The latter method has the potential to detect and quantitate microbial populations.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • Chiral capillary GC, D- and L-alanine, Diaminopimelic acid, Fused silica capillary GC, Mass spectrometry, Muramic acid, Selected ion monitoring, Tuberculostearic acid
Original languageEnglish
Pages (from-to)83-86
JournalChromatographia
Volume16
Issue number1
Publication statusPublished - 1982 Dec 1
Publication categoryResearch
Peer-reviewedYes