Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol

Research output: Contribution to journalArticle

Abstract

Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) < 20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.

Details

Authors
  • Jimmy Rodriguez Murillo
  • Indira Pla
  • Livia Goto-Silva
  • Fábio C.S. Nogueira
  • Gilberto B. Domont
  • Yasset Perez-Riverol
  • Aniel Sánchez
  • Magno Junqueira
Organisations
External organisations
  • Federal University of Rio de Janeiro
  • Wellcome Trust Genome Campus
  • Lund University
  • Skåne University Hospital
  • D'Or Institute for Research and Education
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
  • Cancer and Oncology

Keywords

  • Biological variation, Cell culture, iTRAQ, Quantitative proteomics
Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalAnalytical Biochemistry
Volume559
Publication statusPublished - 2018 Oct 15
Publication categoryResearch
Peer-reviewedYes