Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection

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Abstract

The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biophysics
Original languageEnglish
Pages (from-to)751-756
JournalBiophysical Journal
Volume88
Issue number1
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes