Mechanism of NADH transfer among dehydrogenases

Research output: Contribution to journalArticle

Abstract

Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from one dehydrogenase to another if the two enzymes exhibit distinct chiral coenzyme specificity (A-side vs. B-side). The results lend no support to this proposal, but are fully consistent with a free-diffusion mechanism of NADH transfer irrespective of the chiral specificity of the enzymes.

Details

Authors
  • Wilma Martinez Arias
  • Henrik Pettersson
  • Gösta Pettersson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine

Keywords

  • Dehydrogenase, Channelling, Enzyme kinetics
Original languageEnglish
Pages (from-to)149-156
JournalBBA - Protein Structure and Molecular Enzymology
Volume1385
Issue number1
Publication statusPublished - 1998
Publication categoryResearch
Peer-reviewedYes