Membrane Anchoring and Ion-Entry Dynamics in P-type ATPase Copper Transport

Research output: Contribution to journalArticle

Abstract

Cu+-specific P-type ATPase membrane protein transporters regulate cellular copper levels. The lack of crystal structures in Cu+-binding states has limited our understanding of how ion entry and binding are achieved. Here, we characterize the molecular basis of Cu+ entry using molecular-dynamics simulations, structural modeling, and in vitro and in vivo functional assays. Protein structural rearrangements resulting in the exposure of positive charges to bulk solvent rather than to lipid phosphates indicate a direct molecular role of the putative docking platform in Cu+ delivery. Mutational analyses and simulations in the presence and absence of Cu+ predict that the ion-entry path involves two ion-binding sites: one transient Met148-Cys382 site and one intramembranous site formed by trigonal coordination to Cys384, Asn689, and Met717. The results reconcile earlier biochemical and x-ray absorption data and provide a molecular understanding of ion entry in Cu+-transporting P-type ATPases.

Details

Authors
  • Christina Grønberg
  • Oleg Sitsel
  • Erik Lindahl
  • Pontus Gourdon
  • Magnus Andersson
Organisations
External organisations
  • University of Oxford
  • Stockholm University
  • KTH Royal Institute of Technology
  • University of Copenhagen
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biophysics
  • Medical Biotechnology
Original languageEnglish
Pages (from-to)2417-2429
Number of pages13
JournalBiophysical Journal
Volume111
Issue number11
Publication statusPublished - 2016 Dec 6
Publication categoryResearch
Peer-reviewedYes

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