Method for analysis of methyltetrahydrophthalic acid in urine using gas chromatography and selected ion monitoring

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@article{93ec8e99663d4cd390af8ee47c3c1574,
title = "Method for analysis of methyltetrahydrophthalic acid in urine using gas chromatography and selected ion monitoring",
abstract = "A method for the determination of methyltetrahydrophthalic acid (MTHP acid), a metabolite of methyltetrahydrophthalic anhydride (MTHPA) in human urine, was developed. The investigated MTHP acid was obtained by hydrolysis of a commercial MTHPA mixture, composed of three major isomers. These were synthesized and identified as 3-methyl-delta 4-tetrahydrophthalic anhydride, 4-methyl-delta 4-tetrahydrophthalic anhydride and 4-methyl-delta 3-tetrahydrophthalic anhydride. The urine was worked up by a liquid-solid extraction technique using C18 sorbent columns. Esterification was performed with methanol and boron trifluoride. The derivative in toluene was analyzed with capillary gas chromatography and selected ion monitoring. Deuterium-labeled MTHP acid was used as internal standard. The intra-assay precision for the overall method was between 4 and 8% in the range 3-110 ng/ml and the inter-assay precision was between 4 and 7% in the range 30-110 ng/ml. The total recoveries of the MTHP acid at 19 and 190 ng/ml were 94 and 97%, respectively. The total detection limit for the three isomers was < 6 ng/ml. Analysis of urine from a worker exposed to MTHPA makes it reasonable to assume that the method may be used for biological monitoring of MTHPA exposure.",
author = "Christian Lindh and J{\"o}nsson, {Bo A}",
year = "1994",
doi = "10.1016/0378-4347(94)00271-1",
language = "English",
volume = "660",
pages = "57--66",
journal = "Journal of Chromatography. B",
issn = "1873-376X",
publisher = "Elsevier",
number = "1",

}