Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies

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Abstract

Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.

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Subject classification (UKÄ) – MANDATORY

  • Pediatrics

Keywords

  • Aspiration, biopsy, human, liver, phospholipids, ratio TG/PL, triglycerides
Original languageEnglish
Pages (from-to)53-60
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume26
Issue number1
Publication statusPublished - 1970
Publication categoryResearch
Peer-reviewedYes