Methods to Detect Loss of Lysosomal Membrane Integrity

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on fluorescent dextran release from damaged lysosomes, which can be performed in parallel

Details

Authors
Organisations
External organisations
  • Peter MacCallum Cancer Centre
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell Biology
Original languageEnglish
Title of host publicationAutophagy
Subtitle of host publicationMethods and Protocols
EditorsNicholas Ktistakis, Oliver Florey
Place of PublicationNew York
PublisherHumana Press
Chapter21
Pages315-329
Volume1880
ISBN (Electronic)978149398873-0
Publication statusPublished - 2019 Jan
Publication categoryResearch
Peer-reviewedYes

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

Related projects

Sonja Aits, Kaylene Simpson, Ricky Johnstone, Nikolay Oskolkov & Charlotte Stadler

2016/12/01 → …

Project: Research

View all (1)