Microcontact imprinted plasmonic nanosensors: Powerful tools in the detection of salmonella paratyphi

Research output: Contribution to journalArticle


Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106-15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.


  • Isık Perçin
  • Neslihan Idil
  • Monireh Bakhshpour
  • Erkut Yılmaz
  • Bo Mattiasson
  • Adil Denizli
External organisations
  • Hacettepe University
  • CapSenze HB
  • Aksaray University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
  • Nano Technology


  • Microcontact imprinting, N-methacryloyl-L-histidine methyl ester, Salmonella paratyphi, SPR biosensor
Original languageEnglish
Article number1375
Issue number6
Publication statusPublished - 2017 Jun 13
Publication categoryResearch