Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.

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Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads. / Aragao, Rosa; Alvarez Aliaga, Teresa; Mattiasson, Bo.

In: Biotechnology Letters, Vol. 35, No. 3, 2013, p. 397-405.

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Aragao, Rosa ; Alvarez Aliaga, Teresa ; Mattiasson, Bo. / Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads. In: Biotechnology Letters. 2013 ; Vol. 35, No. 3. pp. 397-405.

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TY - JOUR

T1 - Microcultivation of anaerobic bacteria single cells entrapped in alginate microbeads.

AU - Aragao, Rosa

AU - Alvarez Aliaga, Teresa

AU - Mattiasson, Bo

PY - 2013

Y1 - 2013

N2 - Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose.

AB - Alginate microbeads, produced by emulsion/internal gelation, were studied for the entrapment and microcultivation of microbial cells with biotechnological potential. An anaerobic consortium which was selected for its capacity to degrade complex carbohydrates, and a pure culture of cellulose degrading bacteria were used for entrapment studies. Optimization of conditions for the formation of spherical alginate microbeads in sizes between 20 and 80 μm were examined. The best conditions were achieved by combining rapeseed methyl ester as oil phase and stirring at 100 rpm using a rotation impeller. Calcium alginate microbeads produced under these conditions were shown to present morphological stability, with large pores in the internal matrix that favours microcolony development. Finally, single cells were observed inside the beads after the entrapment procedure and microcolony formation was confirmed after cultivation in cellobiose.

KW - Microcultivation

KW - Single cell

KW - Alginate

KW - Anaerobic bacteria

U2 - 10.1007/s10529-012-1094-1

DO - 10.1007/s10529-012-1094-1

M3 - Article

VL - 35

SP - 397

EP - 405

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 1573-6776

IS - 3

ER -