Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)

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Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae). / Tesson, Sylvie; Borra, Marco; Kooistra, Wiebe; Procaccini, Gabriele.

In: American Journal of Botany, Vol. 98, No. 2, 2011, p. e33-5-e33-5.

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Tesson, Sylvie ; Borra, Marco ; Kooistra, Wiebe ; Procaccini, Gabriele. / Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae). In: American Journal of Botany. 2011 ; Vol. 98, No. 2. pp. e33-5-e33-5.

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TY - JOUR

T1 - Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)

AU - Tesson, Sylvie

AU - Borra, Marco

AU - Kooistra, Wiebe

AU - Procaccini, Gabriele

PY - 2011

Y1 - 2011

N2 - Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction. Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran. Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time.

AB - Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction. Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran. Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time.

KW - microsatellites

KW - mutation accumulation lines

KW - mutation rate.

KW - Mendelian inheritance

KW - diatom

KW - Asexual and sexual reproduction

U2 - 10.3732/ajb.1000430

DO - 10.3732/ajb.1000430

M3 - Article

C2 - 21613102

VL - 98

SP - e33-5-e33-5

JO - American Journal of Botany

JF - American Journal of Botany

SN - 0002-9122

IS - 2

ER -