Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target

Research output: Contribution to journalArticle

Abstract

A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Engineering
  • Analytical Chemistry

Keywords

  • proteomics, spectrometry, mass, SPE, solid-phase extraction, solid-phase microextraction, SPME, integrated selective enrichment target, ISET, MALDI MS
Original languageEnglish
Pages (from-to)1071-1081
JournalJournal of Proteome Research
Volume5
Issue number5
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)