Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target

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Bibtex

@article{1ddd40abae06466cadf233114de9daed,
title = "Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target",
abstract = "A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.",
keywords = "proteomics, spectrometry, mass, SPE, solid-phase extraction, solid-phase microextraction, SPME, integrated selective enrichment target, ISET, MALDI MS",
author = "Simon Ekstr{\"o}m and Lars Wallman and D Hok and Gy{\"o}rgy Marko-Varga and Thomas Laurell",
note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)",
year = "2006",
doi = "10.1021/pr050434z",
language = "English",
volume = "5",
pages = "1071--1081",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "The American Chemical Society (ACS)",
number = "5",

}