MiR-155-5p controls colon cancer cell migration via post-transcriptional regulation of Human Antigen R (HuR)

Research output: Contribution to journalArticle

Abstract

Colorectal cancer (CRC) is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the worst prognostic factor for patients with CRC. HuR (ELAVL1) is overexpressed in CRC and has been reported to promote colon cancer growth by targeting RNA in the cell cytoplasm. Herein, the role of miR-155-5p in regulating HuR expression and cell migration was examined in colon cancer cells. MiR-155-5p knockdown in serum-starved colon cancer cells decreased both colon cancer cell chemotaxis and cytoplasmic expression of HuR. Bioinformatics analysis predicted two putative binding sites in the AU-rich elements (AREs) at the 3′-UTR of HuR mRNA. MiR-155-5p binding to HuR was verified using specific target site blockers and functionally validated by use of RNA immunoprecipitation assays, showing that miR-155-5p-dependent regulation of HuR expression is mediated by AREs. Targeting AREs with a specific blocker inhibited colon cancer cell migration. Taken together, these novel findings demonstrate that AREs mediate miR-155-5p positive regulation of HuR mRNA levels and translation as well as migration in colon cancer cells, suggesting that targeting miR-155-5p and/or Hur might be useful therapeutic strategies against colon cancer metastasis.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
  • Surgery

Keywords

  • Cell migration, Colon cancer, HuR, Metastasis, microRNA
Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalCancer Letters
Volume421
Publication statusPublished - 2018 May 1
Publication categoryResearch
Peer-reviewedYes

Related research output

Amr Al-Haidari, 2018, Lund: Lund University, Faculty of Medicine. 78 p.

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