MiR-335 regulates exocytotic proteins and affects glucose-stimulated insulin secretion through decreased Ca2+-dependent exocytosis in beta cells

Research output: Contribution to journalPublished meeting abstract

Abstract

Background and aims: Ca2+-induced exocytosis is essential for insulin to be secreted from beta-cells, and in islets from type-2 diabetic (T2D) donors the expression of several genes coding for exocytotic proteins is reduced. Largely this phenomenon cannot be explained by polymorphism; rather it is likely due to epigenetic factors like microRNAs (miRNAs). Indeed, previous studies have identified a number of miRNAs with differential expression in the islets from T2D donors and the Goto- Kakizaki (GK) rat. One of the upregulatedmiRNAs in the GK rat is miR- 335, predicted to target several exocytotic genes amongst those Stxbp1 is a validated target. Here we aim to investigate whether miR-335 regulates the expression of exocytotic genes and affects insulin secretion and exocytosis in beta-cells. Materials and methods: Insulin secretion was measured by radio immuno assay. Exocytosis and docking of insulin granules was studied by capacitance measurements using the patch-clamp technique and by TIRF microscopy. Rat miR-335 was overexpressed using chemicallymodified mature microRNA mimic in INS-1 832/13 beta-cells by transfection. Gene knockdown was performed with RNAi. Protein and mRNA levels were analysed with Western Blot and RT-qPCR, respectively. Results: Overexpression of miR-335 (OE335) in INS-1 832/13 cells reduced insulin secretion at 16.7 mM glucose compared to control cells (SCR) (n=3; p

Details

Authors
Organisations
External organisations
  • Uppsala University
  • Lund University
  • Roskilde University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes

Keywords

  • glucose, protein, insulin, microRNA, synaptotagmin, nitrogen 15, messenger RNA, insulin release, exocytosis, European, diabetes mellitus, gene, microscopy, donor, membrane, Goto Kakizaki rat, rat, genetic transfection, patch clamp technique, immunoassay, gene silencing, pancreas islet beta cell, density, electric potential, Western blotting, adaptation, cell function, telecommunication
Original languageEnglish
Pages (from-to)S128
Number of pages1
JournalDiabetologia
Volume58
Issue numberSuppl. 1
Publication statusPublished - 2015 Sep 1
Publication categoryResearch
Peer-reviewedYes