Model cellulose films exposed to H-insolens glucoside hydrolase family 45 endo-cellulase - the effect of the carbohydrate-binding module

Research output: Contribution to journalArticle

Abstract

The effects of enzyme structure and activity on the degradation of model cellulose substrates were investigated by ellipsometry for the cellulase Humicola insolens GH45. The inactive variant D10N was found to adsorb at the cellulose surface but also to be incorporated into the cellulose films to an extent that depended on pH. For the native protein, the initial adsorption monitored for the inactive variant D10N was followed by enzyme-mediated degradation of the cellulose films. Again, a dependence on pH was found, such that higher pH resulted in slower enzymatic degradation. Removing the carbohydrate-binding module eliminated this pH dependence but also resulted in a decreased adsorption to the cellulose surface, and in a decreased net catalytic effect. (c) 2004 Elsevier Inc. All rights reserved.

Details

Authors
  • Jonny Eriksson
  • M Malmsten
  • Fredrik Tiberg
  • T H Callisen
  • T Damhus
  • K S Johansen
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Physical Chemistry
Original languageEnglish
Pages (from-to)94-99
JournalJournal of Colloid and Interface Science
Volume285
Issue number1
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes