Modeling and optimization of preparative reversed-phase liquid chromatography for insulin purification

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Modeling and optimization of preparative reversed-phase liquid chromatography for insulin purification. / Degerman, Marcus; Jakobsson, Niklas; Nilsson, Bernt.

In: Journal of Chromatography A, Vol. 1162, No. 1, 2007, p. 41-49.

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TY - JOUR

T1 - Modeling and optimization of preparative reversed-phase liquid chromatography for insulin purification

AU - Degerman, Marcus

AU - Jakobsson, Niklas

AU - Nilsson, Bernt

PY - 2007

Y1 - 2007

N2 - This paper presents a model for reversed-phase purification of insulin from desamido insulin. The system is described by a reaction dispersive model with a competitive Langmuir isotherm. A model building and calibration method is presented and the model's region of validity is defined. The model is calibrated using only two-component experiments on the raw mixture by the inverse method and then experimentally validated. The model is then used to optimize the system's production rate with both purity and yield requirements. The yield requirement is varied between 80 and 95% to study the effect on the production rate and the operating point. The operating points found with the optimization were found outside the model's region of validity, but the experimental validation of the operating points shows that the model can be extrapolated to the interesting operating points. (c) 2007 Elsevier B.V. All rights reserved.

AB - This paper presents a model for reversed-phase purification of insulin from desamido insulin. The system is described by a reaction dispersive model with a competitive Langmuir isotherm. A model building and calibration method is presented and the model's region of validity is defined. The model is calibrated using only two-component experiments on the raw mixture by the inverse method and then experimentally validated. The model is then used to optimize the system's production rate with both purity and yield requirements. The yield requirement is varied between 80 and 95% to study the effect on the production rate and the operating point. The operating points found with the optimization were found outside the model's region of validity, but the experimental validation of the operating points shows that the model can be extrapolated to the interesting operating points. (c) 2007 Elsevier B.V. All rights reserved.

KW - downstream processing

KW - model calibration

KW - optimization

KW - modeling

KW - insulin

KW - reversed phase

U2 - 10.1016/j.chroma.2007.02.062

DO - 10.1016/j.chroma.2007.02.062

M3 - Article

VL - 1162

SP - 41

EP - 49

JO - Journal of chromatography. A

JF - Journal of chromatography. A

SN - 0021-9673

IS - 1

ER -