Modulation of epileptogenesis: A paradigm for the integration of enzyme-based microelectrode arrays and optogenetics

Research output: Contribution to journalArticle

Abstract

Background
Genesis of acquired epilepsy includes transformations spanning genetic-to- network-level modifications, disrupting the regional excitatory/inhibitory balance. Methodology concurrently tracking changes at multiple levels is lacking. Here, viral vectors are used to differentially express two opsin proteins in neuronal populations within dentate gyrus (DG) of hippocampus. When activated, these opsins induced excitatory or inhibitory neural output that differentially affected neural networks and epileptogenesis. In vivo measures included behavioral observation coupled to real-time measures of regional glutamate flux using ceramic-based amperometric microelectrode arrays (MEAs).

Details

Authors
  • Corwin R. Butler
  • Jeffery A. Boychuk
  • Francois Pomerleau
  • Ramona Alcala
  • Peter Huettl
  • Yi Ai
  • Johan Jakobsson
  • Sidney W. Whiteheart
  • Greg A. Gerhardt
  • Bret N. Smith
  • John T Slevin
Organisations
External organisations
  • University of Kentucky
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences
Original languageEnglish
Article number106244
JournalEpilepsy Research
Volume159
Publication statusPublished - 2020 Jan 20
Publication categoryResearch
Peer-reviewedYes