Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C

Research output: Contribution to journalArticle


Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences


  • Enzyme inhibitor, Cysteine proteinase, Cerebral hemorrhage, Cystatin C, cDNA cloning, Nucleotide sequence
Original languageEnglish
Pages (from-to)229-233
JournalFEBS Letters
Issue number2
Publication statusPublished - 1987
Publication categoryResearch