Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C

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Abstract

Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.

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Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Enzyme inhibitor, Cysteine proteinase, Cerebral hemorrhage, Cystatin C, cDNA cloning, Nucleotide sequence
Original languageEnglish
Pages (from-to)229-233
JournalFEBS Letters
Volume216
Issue number2
Publication statusPublished - 1987
Publication categoryResearch
Peer-reviewedYes