Molecularly imprinted microspheres as antibody binding mimics

Research output: Contribution to journalArticle

Abstract

Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Industrial Biotechnology

Keywords

  • Enzyme-linked immunosorbent assay, Immunoassay, Microspheres, Molecular imprinting, Precipitation polymerization
Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalReactive and Functional Polymers
Volume48
Issue number1-3
Publication statusPublished - 2001 May 2
Publication categoryResearch
Peer-reviewedYes
Event9th International Conference on Polymer-Based Technology - , China
Duration: 2000 May 212000 May 21