Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood

Research output: Contribution to journalArticle

Abstract

A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity.

Details

Authors
  • Ali Derazshamshir
  • Goezde Baydemir
  • Muege Andac
  • Ridvan Say
  • Igor Galaev
  • Adil Denizli
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • hydrogels, hemoglobin depletion, affinity binding, cryogels, molecular, recognition
Original languageEnglish
Pages (from-to)657-668
JournalMacromolecular Chemistry and Physics
Volume211
Issue number6
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes