Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood

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Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood. / Derazshamshir, Ali; Baydemir, Goezde; Andac, Muege; Say, Ridvan; Galaev, Igor; Denizli, Adil.

In: Macromolecular Chemistry and Physics, Vol. 211, No. 6, 2010, p. 657-668.

Research output: Contribution to journalArticle

Harvard

Derazshamshir, A, Baydemir, G, Andac, M, Say, R, Galaev, I & Denizli, A 2010, 'Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood', Macromolecular Chemistry and Physics, vol. 211, no. 6, pp. 657-668. https://doi.org/10.1002/macp.200900425

APA

Derazshamshir, A., Baydemir, G., Andac, M., Say, R., Galaev, I., & Denizli, A. (2010). Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood. Macromolecular Chemistry and Physics, 211(6), 657-668. https://doi.org/10.1002/macp.200900425

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MLA

Vancouver

Author

Derazshamshir, Ali ; Baydemir, Goezde ; Andac, Muege ; Say, Ridvan ; Galaev, Igor ; Denizli, Adil. / Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood. In: Macromolecular Chemistry and Physics. 2010 ; Vol. 211, No. 6. pp. 657-668.

RIS

TY - JOUR

T1 - Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood

AU - Derazshamshir, Ali

AU - Baydemir, Goezde

AU - Andac, Muege

AU - Say, Ridvan

AU - Galaev, Igor

AU - Denizli, Adil

PY - 2010

Y1 - 2010

N2 - A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity.

AB - A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity.

KW - hydrogels

KW - hemoglobin depletion

KW - affinity binding

KW - cryogels

KW - molecular

KW - recognition

U2 - 10.1002/macp.200900425

DO - 10.1002/macp.200900425

M3 - Article

VL - 211

SP - 657

EP - 668

JO - Macromolecular Chemistry and Physics

JF - Macromolecular Chemistry and Physics

SN - 1521-3935

IS - 6

ER -