mTHPC pharmacokinetics following topical administration

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20 μl/cm<sup>2</sup> of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumor area and the concentration of mTHPC was measured at 4 and 6 hours after drug application by two methods: 1. A fluorescence imaging system capturing images at two wavelengths (500 and 650 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 650 to 500 was calculated. 2. A diffuse reflectance spectroscopy system with a fiber separation of 2 mm, providing the absorbance at 652 nm. Both systems provided consistent results related to the photosensitizer concentration. The methods show a remarkable difference in the concentration of photosensitizer in normal skin and tumor. No significant difference in mTHPC concentration in tumor could be observed between the 4 and 6h groups after drug application.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Dermatology and Venereal Diseases

Keywords

  • MTHPC, Skin carcinomas, Fluorescence imaging, Fluorescence spectroscopy
Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing VI
PublisherInternational Society for Optical Engineering
PagesC940-C940
Volume6094
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes
EventOptical Diagnostics and Sensing VI - San Jose, CA, United States
Duration: 2006 Jan 242006 Jan 25

Publication series

Name
Volume6094
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOptical Diagnostics and Sensing VI
CountryUnited States
CitySan Jose, CA
Period2006/01/242006/01/25

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