Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities

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Bibtex

@article{e07ef71f823848c89977fcd8b9abb90e,
title = "Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities",
abstract = "The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.",
keywords = "Pheromone biosynthesis, Spodoptera exigua, Spodoptera litura, ∆11 desaturation, ∆12 desaturation",
author = "Xia, {Yi Han} and Zhang, {Ya Nan} and Ding, {Bao Jian} and Wang, {Hong Lei} and Christer L{\"o}fstedt",
year = "2019",
doi = "10.1007/s10886-019-01067-3",
language = "English",
volume = "45",
pages = "378--387",
journal = "Journal of Chemical Ecology",
issn = "1573-1561",
publisher = "Springer",
number = "4",

}