Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities

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T1 - Multi-Functional Desaturases in Two Spodoptera Moths with ∆11 and ∆12 Desaturation Activities

AU - Xia, Yi Han

AU - Zhang, Ya Nan

AU - Ding, Bao Jian

AU - Wang, Hong Lei

AU - Löfstedt, Christer

PY - 2019

Y1 - 2019

N2 - The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.

AB - The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ∆12 desaturase activity involved in the biosynthesis of this compound; however, the putative ∆12 desaturase gene has not been reported to date. In the present study, we confirmed this ∆12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions.

KW - Pheromone biosynthesis

KW - Spodoptera exigua

KW - Spodoptera litura

KW - ∆11 desaturation

KW - ∆12 desaturation

U2 - 10.1007/s10886-019-01067-3

DO - 10.1007/s10886-019-01067-3

M3 - Article

VL - 45

SP - 378

EP - 387

JO - Journal of Chemical Ecology

T2 - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 1573-1561

IS - 4

ER -