Multimodal imaging to study the morphochemistry of basal cell carcinoma

Research output: Contribution to journalArticle

Abstract

Basal cell carcinoma is the most abundant malignant neoplasm in humans, the pathology of which is characterized by an abnormal proliferation of basal cells. Basal cell carcinoma can show a variety of different morphologies, which are based on different cellular biology. Furthermore, the carcinoma often grows invisibly to the eye imbedded in the surrounding skin. Therefore, in some cases its clinical detection is challenging. Thus, our work aims at establishing an unsupervised tissue classification method based on multimodal imaging and the application of chemometrics to discriminate basal cell carcinoma from non-diseased tissue. A case study applying multimodal imaging to ex-vivo sections of basal cell carcinoma is presented. In doing so, we apply a combination of various linear and non-linear imaging modalities, i.e. fluorescence, Raman and second-harmonic generation microscopy, to study the morphochemistry of basal cell carcinoma. The joint information content obtained by such multimodal approach in studying various aspects of the malignant tissue alterations associated with basal cell carcinoma is discussed. [GRAPHICS] Multimodal imaging combining coherent anti-Stokes Raman scattering, second-harmonic generation and two-photo fluorescence is combined with Raman spectroscopy to investigate the morphochemistry of human basal cell carcinoma.

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Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
  • Dermatology and Venereal Diseases
Original languageEnglish
Pages (from-to)728-736
JournalJournal of Biophotonics
Volume3
Issue number10-11
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Atomic physics (011013005), Department of Dermatology and Venerology (013241320), Oncology, MV (013035000), Department of Dermatology and Venereology (Lund) (013006000)