Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4

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Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4. / Duncan, Renee C.; Mohlin, Frida; Coetzer, Theresa H.; Blom, Anna; Wijeyewickrema, Lakshmi C.; Pike, Robert N.

In: Molecular Immunology, Vol. 49, No. 4, 2012, p. 593-600.

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Duncan, Renee C. ; Mohlin, Frida ; Coetzer, Theresa H. ; Blom, Anna ; Wijeyewickrema, Lakshmi C. ; Pike, Robert N. / Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4. In: Molecular Immunology. 2012 ; Vol. 49, No. 4. pp. 593-600.

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TY - JOUR

T1 - Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4

AU - Duncan, Renee C.

AU - Mohlin, Frida

AU - Coetzer, Theresa H.

AU - Blom, Anna

AU - Wijeyewickrema, Lakshmi C.

AU - Pike, Robert N.

PY - 2012

Y1 - 2012

N2 - The complement system is fundamental to both innate and adaptive immunity and can be initiated via the classical, lectin or alternative pathways. Cleavage of C4 by MASP-2, the initiating protease of the lectin pathway, is a crucial event in the activation of this pathway, preceding the eventual formation of the C3 convertase (C4bC2a) complex on the pathogen surface. Interactions required for the cleavage of C4 by MASP-2 are likely to be facilitated by the initial binding of C4 to an exosite on the protease. We have shown that both proteolytically active and catalytically inactive CCP1-CCP2-serine protease (CCP1-CCP2-SP) forms bind C4 with similar affinity. Interestingly, proteins containing the CCP1-CCP2 domains or the SP domain alone bound C4 with much lower affinity than the CCP1-CCP2-SP protein, suggesting that the CCP domains cooperate positively with the active site to mediate efficient binding and cleavage of C4. In addition, mutation of residue K342 to alanine in the CCP1 domain abolished binding to both C4 and C4b in its CCP1-CCP2 form, suggesting a key electrostatic role for this amino acid. The presented data indicates that all of the domains are required in order to mediate high affinity interaction with C4. (C) 2011 Elsevier Ltd. All rights reserved.

AB - The complement system is fundamental to both innate and adaptive immunity and can be initiated via the classical, lectin or alternative pathways. Cleavage of C4 by MASP-2, the initiating protease of the lectin pathway, is a crucial event in the activation of this pathway, preceding the eventual formation of the C3 convertase (C4bC2a) complex on the pathogen surface. Interactions required for the cleavage of C4 by MASP-2 are likely to be facilitated by the initial binding of C4 to an exosite on the protease. We have shown that both proteolytically active and catalytically inactive CCP1-CCP2-serine protease (CCP1-CCP2-SP) forms bind C4 with similar affinity. Interestingly, proteins containing the CCP1-CCP2 domains or the SP domain alone bound C4 with much lower affinity than the CCP1-CCP2-SP protein, suggesting that the CCP domains cooperate positively with the active site to mediate efficient binding and cleavage of C4. In addition, mutation of residue K342 to alanine in the CCP1 domain abolished binding to both C4 and C4b in its CCP1-CCP2 form, suggesting a key electrostatic role for this amino acid. The presented data indicates that all of the domains are required in order to mediate high affinity interaction with C4. (C) 2011 Elsevier Ltd. All rights reserved.

KW - Protease

KW - Mannan-binding lectin-associated serine protease-2

KW - C4

KW - Exosite

U2 - 10.1016/j.molimm.2011.10.006

DO - 10.1016/j.molimm.2011.10.006

M3 - Article

VL - 49

SP - 593

EP - 600

JO - Immunochemistry

T2 - Immunochemistry

JF - Immunochemistry

SN - 1872-9142

IS - 4

ER -