Multiple gene genealogies and species recognition in the ectomycorrhizal fungus Paxillus involutus.

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Multiple gene genealogies and species recognition in the ectomycorrhizal fungus Paxillus involutus. / Hedh, Jenny; Samson, Peter; Erland, Susanne; Tunlid, Anders.

In: Mycological Research, Vol. 112, No. 8, 2008, p. 965-975.

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Hedh, Jenny ; Samson, Peter ; Erland, Susanne ; Tunlid, Anders. / Multiple gene genealogies and species recognition in the ectomycorrhizal fungus Paxillus involutus. In: Mycological Research. 2008 ; Vol. 112, No. 8. pp. 965-975.

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TY - JOUR

T1 - Multiple gene genealogies and species recognition in the ectomycorrhizal fungus Paxillus involutus.

AU - Hedh, Jenny

AU - Samson, Peter

AU - Erland, Susanne

AU - Tunlid, Anders

PY - 2008

Y1 - 2008

N2 - Paxillus involutus (basidiomycetes, Boletales) is a common ectomycorrhizal fungus in the Northern Hemisphere. The fungus displays significant variation in phenotypic characters related to morphology, physiology, and ecology. Previous studies have shown that P. involutus contains several intersterility groups and morphological species. In this study, we have used concordance of multiple gene genealogies to identify genetically isolated species of P. involutus. Fragments from five protein coding genes in 50 isolates of P. involutus collected from different hosts and environments in Europe and one location in Canada were analysed using phylogenetic methods. Concordance of the five gene genealogies showed that P. involutus comprises at least four distinct phylogenetic lineages: phylogenetic species I (with nine isolates), II (33 isolates), III (three isolates), and IV (five isolates). The branches separating the four species were long and well supported compared with the species internodes. A low level of shared polymorphisms was observed among the four lineages indicating a long time since the genetic isolation began. Three of the phylospecies corresponded to earlier identified morphological species: I to P. obscurosporus, II to P. involutus s. str., and III to P. validus. The phylogenetic species had an overlapping geographical distribution. Species I and II differed partly in habitat and host preferences.

AB - Paxillus involutus (basidiomycetes, Boletales) is a common ectomycorrhizal fungus in the Northern Hemisphere. The fungus displays significant variation in phenotypic characters related to morphology, physiology, and ecology. Previous studies have shown that P. involutus contains several intersterility groups and morphological species. In this study, we have used concordance of multiple gene genealogies to identify genetically isolated species of P. involutus. Fragments from five protein coding genes in 50 isolates of P. involutus collected from different hosts and environments in Europe and one location in Canada were analysed using phylogenetic methods. Concordance of the five gene genealogies showed that P. involutus comprises at least four distinct phylogenetic lineages: phylogenetic species I (with nine isolates), II (33 isolates), III (three isolates), and IV (five isolates). The branches separating the four species were long and well supported compared with the species internodes. A low level of shared polymorphisms was observed among the four lineages indicating a long time since the genetic isolation began. Three of the phylospecies corresponded to earlier identified morphological species: I to P. obscurosporus, II to P. involutus s. str., and III to P. validus. The phylogenetic species had an overlapping geographical distribution. Species I and II differed partly in habitat and host preferences.

KW - ITS sequences

KW - Cryptic species

KW - Boletales

KW - Mycorrhizas

KW - Phylogenetic species Species concepts

U2 - 10.1016/j.mycres.2008.01.026

DO - 10.1016/j.mycres.2008.01.026

M3 - Article

VL - 112

SP - 965

EP - 975

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

IS - 8

ER -