Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture

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Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture. / Sekula, Sylwia; Fuchs, Jeanette; Weg-Remers, Susanne; Nagel, Peter; Schuppler, Stefan; Fragala, Joe; Theilacker, Nora; Franueb, Matthias; Wingren, Christer; Ellmark, Peter; Borrebaeck, Carl; Mirkin, Chad A.; Fuchs, Harald; Lenhert, Steven.

In: Small, Vol. 4, No. 10, 2008, p. 1785-1793.

Research output: Contribution to journalArticle

Harvard

Sekula, S, Fuchs, J, Weg-Remers, S, Nagel, P, Schuppler, S, Fragala, J, Theilacker, N, Franueb, M, Wingren, C, Ellmark, P, Borrebaeck, C, Mirkin, CA, Fuchs, H & Lenhert, S 2008, 'Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture', Small, vol. 4, no. 10, pp. 1785-1793. https://doi.org/10.1002/smll.200800949

APA

Sekula, S., Fuchs, J., Weg-Remers, S., Nagel, P., Schuppler, S., Fragala, J., ... Lenhert, S. (2008). Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture. Small, 4(10), 1785-1793. https://doi.org/10.1002/smll.200800949

CBE

Sekula S, Fuchs J, Weg-Remers S, Nagel P, Schuppler S, Fragala J, Theilacker N, Franueb M, Wingren C, Ellmark P, Borrebaeck C, Mirkin CA, Fuchs H, Lenhert S. 2008. Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture. Small. 4(10):1785-1793. https://doi.org/10.1002/smll.200800949

MLA

Vancouver

Author

Sekula, Sylwia ; Fuchs, Jeanette ; Weg-Remers, Susanne ; Nagel, Peter ; Schuppler, Stefan ; Fragala, Joe ; Theilacker, Nora ; Franueb, Matthias ; Wingren, Christer ; Ellmark, Peter ; Borrebaeck, Carl ; Mirkin, Chad A. ; Fuchs, Harald ; Lenhert, Steven. / Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture. In: Small. 2008 ; Vol. 4, No. 10. pp. 1785-1793.

RIS

TY - JOUR

T1 - Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture

AU - Sekula, Sylwia

AU - Fuchs, Jeanette

AU - Weg-Remers, Susanne

AU - Nagel, Peter

AU - Schuppler, Stefan

AU - Fragala, Joe

AU - Theilacker, Nora

AU - Franueb, Matthias

AU - Wingren, Christer

AU - Ellmark, Peter

AU - Borrebaeck, Carl

AU - Mirkin, Chad A.

AU - Fuchs, Harald

AU - Lenhert, Steven

PY - 2008

Y1 - 2008

N2 - Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells.

AB - Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells.

KW - proteomics

KW - phospholipids

KW - dip-pen nanolithography

KW - biomimetics

KW - cell adhesion

U2 - 10.1002/smll.200800949

DO - 10.1002/smll.200800949

M3 - Article

VL - 4

SP - 1785

EP - 1793

JO - Small

T2 - Small

JF - Small

SN - 1613-6829

IS - 10

ER -